The state government is cutting WorkCover compensation payments to some public service staff, including cover for injuries sustained on the way to and from work.
Nurses and other hospital staff are among those facing cutbacks, but firefighters have won exemptions following last month’s strikes. Police are also exempt.
Opposition leader John Robertson said he hoped to get 10,000 signatures on the petition to force the government to debate the laws again.
“This legislation has been passed, but that doesn’t mean this fight is over,” he said.
“We’re aiming to get more than 10,000 signatures on this petition so we can see a debate,” Mr Robertson added.
“The O’Farrell government needs to stand up in the parliament and justify these changes.”
Mr Robertson suggested some government MPs were uncomfortable about the cutbacks when they were last debated.
The government last year promised to debate any subject raised in a petition to parliament carrying 10,000 or more signatures.